EARLY-STAGE BIOINFORMATICS COMPANY
Kmeggie+SEQUENCING, SIMPLIFIED

THE PLATFORM

Quality control that starts where the data lives.

Kmeggie+ brings FASTQ validation, understandable QC reporting, and a CLI-first workflow into a lightweight local toolkit.

Working prototype In development Planned
01
WORKING PROTOTYPE

FASTQ validation

Catch structural problems before downstream analysis.

The current analyzer checks record structure, supported DNA bases, the separator line, quality characters, and whether sequence and quality lengths agree.

  • Four-line FASTQ records
  • Non-empty read headers
  • A, C, G, T, and N sequences
  • Matching sequence and quality lengths
  • Printable Phred+33 quality scores
$ validator result: example_500_reads.fastqstatus              VALID
reads               500
total bases         75,000
average read length 150 bp

next                REVIEW QUALITY REPORT
02
BROWSER PROTOTYPE

Automated QC reporting

Turn measurements into a report a laboratory team can review.

The browser prototype analyzes every accepted read within its limits and presents base quality, base composition, read lengths, duplicate fraction, N content, and simplified validation flags.

Open the live QC demo
KMEGGIE+ QC RECORDSYNTHETIC SAMPLE
Total reads
500
Total bases
75,000
Mean quality
30.0
Q30 bases
66.7%

Finding: FASTQ structure is valid. The final 50 base positions need review.

03
EARLY CLI PROTOTYPE

CLI-first workflow

A local workflow before an institutional platform.

The working local prototype can be installed in a Python environment and run on a laboratory machine. The public package and a complete orchestration interface are not yet released.

Use the current quickstart → 
$ current local prototypeunzip kmeggie-prototype.zip
cd kmeggie-prototype
python3 -m venv venv
source venv/bin/activate
pip install -r requirements.txt
python app.py

# open http://127.0.0.1:5000

PROPOSED ARCHITECTURE

A path toward asynchronous processing.

This diagram is a design proposal for submitting, tracking, processing, and reporting pipeline jobs. It does not describe a released system.

Proposed Kmeggie+ asynchronous FASTQ pipeline architecture
Architecture proposal · classification, queues, shared storage, and institutional deployment remain planned.

PRODUCT ROADMAP

Clear stages, clear claims.

Capability Stage What that means
FASTQ structural validation Working Implemented in functional prototypes.
Browser QC analysis and export Working Available in the live website demo.
CLI packaging and orchestration Developing Local prototype exists; fuller pipeline integration is underway.
Metagenomic classification Planned Optional downstream stage after validation and QC.
Multi-lab dashboard Planned Institutional monitoring and comparable reporting.
AI-assisted variant triage Planned Future decision support; no released implementation.

EVALUATE THE WORK

Run the demo or discuss a pilot.

Try the QC demoRequest a pilot