EARLY-STAGE BIOINFORMATICS COMPANY
Kmeggie+SEQUENCING, SIMPLIFIED

DOCUMENTATION

Run the current prototype locally.

This quickstart reflects the working local Flask prototype. A public pip install package and CLI repository are not yet published here.

01 / REQUIREMENTS

Before you begin

  • Python 3
  • A copy of kmeggie-prototype.zip supplied for evaluation
  • A local machine with enough space for the FASTQ files you intend to inspect

The current package is shared directly with evaluators; there is no verified public download link on this site.

02 / LOCAL QUICKSTART

Install and start the prototype

$ create an isolated Python environmentunzip kmeggie-prototype.zip
cd kmeggie-prototype
python3 -m venv venv
source venv/bin/activate
pip install -r requirements.txt
python app.py

When the local server starts, open http://127.0.0.1:5000 in the same machine’s browser.

Current architecture note

The local Flask QC engine and the CLI validators are prototype components. Their complete unified workflow is still in development.

03 / NO-INSTALL DEMO

Use the website prototype

  1. Open the QC workspace.
  2. Run the 500-read synthetic sample or choose a supported local FASTQ file.
  3. Review validation checks, metrics, and charts.
  4. Export the report as HTML.
Open the live QC demo

04 / SUPPORTED INPUT

Prototype limits

Files .fastq, .fq, and gzip-compressed variants
Records Four-line, single-end FASTQ
Quality encoding Phred+33
Upload size 50 MB
Decompressed size 100 MB
Read count 200,000 reads
Read length 100,000 bases per read

Files above a limit are rejected rather than sampled. Adapter detection, paired-end validation, and classification are not included.

05 / RELEASE STATUS

What is published

PUBLICBrowser QC demoOpen →
DIRECT ACCESSLocal prototype packageShared with evaluators
PENDINGPublic CLI packageNo pip install kmeggie claim yet
PENDINGPublic CLI source repositoryLink will appear after publication